Phoneutria boliviensis is a species of a medically important spider in the family Ctenidae, found in Central and South America.

Male Males have a varied color, prosome, legs and sternum brown, dark opistomatic. Males grow from 30 to 35 mm in length. '''Females The females are slightly larger, growing up to 30-40 mm in length, with a yellowish to brownish brown prosoma, with a thin longitudinal black band, lateral black lines on the clipped, and dorsally on the pedipalp with yellow bands. The opistogosome is dorsally yellowish, with beams to pair with a bright yellow-brownish pair, in the ventral part it varies from gold to yellow with two sharp brown spots and two white spots, have cheliceras that vary from red to reddish-brown, dorsally the legs have two small yellow spots and thin black transverse stripes.

Phoneutria boliviensis lives in both Central and South America, in South America it can be seen in Bolivia, Paraguay, Peru, Ecuador, Brazil and Colombia, are found in habitats associated with dry and humid tropical forests, usually in soil with little waste.

Females lay up to 4 egg sacks, with 430-1,300 puppies hatching after 28-34 days. Reproductive behavior consists of the male riding on the female, showing a typical copulation position of modern wandering spiders.